University of Sadat City, Genetic Engineering & Biotechnology Research Institute (GEBRI)Research Journal of Applied Biotechnology2356-94332Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology20161101MTHFR A1298C POLYMORPHISM AND THE VASO-OCCLUSIVE CRISS IN SICKLE CELL DISEASE165936510.21608/rjab.2016.59365ENAhmed A.RaiufClinical Biochemistry Department, National Liver Institute, Menoufia University, EgyptMona M.HamdyPediatrics Department, Faculty of Medicine, Cairo University, EgyptOsama F.ShalaanMolecular Diagnostics and Therapeutics Department, Genetic Engineering and
Biotechnology Research Institute, University of Sadat City, EgyptMoustafa A.SakrMolecular Diagnostics and Therapeutics Department, Genetic Engineering and
Biotechnology Research Institute, University of Sadat City, EgyptAbdel Rahman A.Abdel RahmanMolecular Diagnostics & Therapeutics Department, Genetic Engineering and Biotechnology Research Institute, University Of Sadat City, Sadat city, Egypt.Journal Article20191115Vaso-occlusion is the most signs and symptoms of sickle-cell anemia (SCA). Elevated Homocysteine concentration contributes to form thrombosis, a frequent event in sickle cell anemia. Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme, which modifies homocysteine metabolism, and some polymorphisms of gene encoding this enzyme are accompanied with a decreased activity of the enzyme. The aim of the study was to study the association between the A1298C polymorphism and the incidence of Vaso-occlusive crisis. A case- control study was conducted over a period of one year from Jan- Dec 2014 inclusive, 50 patients were collected together with age, and sex matched healthy control 30 cases. Venous blood samples were collected from groups to estimate serum Homocysteine, folic acid and A1298C polymorphism identification through tetra primer ARMS PCR. Statistical analysis was done, using the student t-test, Pearson correlation analysis and χ2 test. We found that, the homocysteine level was significantly upregulated in the patients group compared with control group with p value > 0.01. Moreover, a strong positive correlation between Homocysteine level and the frequency of Vaso-occlusive crisis was found (χ2= 4.836 and p value 0.04). Association between vaso-occlusive events and polymorphism frequency showed a non-significant difference for the A1298C gene (χ2 = 1.720; p = 0.4231). We conclude that Hyperhomocysteinaemia is positively correlated with the frequency of Vaso occlusive crisis and the presence of A1298C MTHFR gene polymorphism is not a risk factor for vaso-occlusive crisis in the SCD patients.https://rjab.journals.ekb.eg/article_59365_7992b2ff3aabedc97566ee7094734b44.pdfUniversity of Sadat City, Genetic Engineering & Biotechnology Research Institute (GEBRI)Research Journal of Applied Biotechnology2356-94332Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology20161101THE EFFECT OF PURIFIED NIGELLA SATIVA LECTIN AGAINST TUMOR DEVELOPMENT IN FEMALE ALBINO MICE AND GENETIC POLYMORPHISM IN PUMA GENE7185937110.21608/rjab.2016.59371ENWael Abdel-MageedMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat city, Sadat City, Egypt.Marwa MareyMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat city, Sadat City, Egypt.Khalil El-HalfawyMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat city, Sadat City, Egypt.Journal Article20191115The purpose of this study examine whether the polymorphism in p53 upregulated modulator of apoptosis (PUMA) gene consequential treated with purified Nigella sativa lectin in tumor induced mice. Nigella sativa (N. sativa) is a widely used medicinal plant throughout the world. Possess a seed lectin that was purified by combination of ammonium sulfate precipitation and affinity chromatography on a Sepharose 4B column. The Nigella sativa strongly agglutinated human erythrocytes. The hemagglutinating activity of lectin was maintained after incubation at a wide range of temperature and pH and also was independent of divalent cations. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, lectin exhibited an electrophoretic profile consisting of a single band with apparent molecular mass of 45 kDa. Experimental groups were organized into 6 groups including 10 animals per each, additionally control group, blood samples were collected form mice before and after lectin treatment for liver and kidney enzymes biochemical analysis, Data statistical analysis for mitochondrial enzymes in our study showed a very highly significant (P>0.001) difference in the mean value of serum enzymes isolated from treatments groups with the polymorphism of the PUMA gene were typed from genomic DNA, the results from PUMA showed a strong deferent between the treatments with lectin.https://rjab.journals.ekb.eg/article_59371_81aac6e4e1be150198863864360f94ae.pdfUniversity of Sadat City, Genetic Engineering & Biotechnology Research Institute (GEBRI)Research Journal of Applied Biotechnology2356-94332Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology20161101ASSOCIATION BETWEEN POLYMORPHISMS OF THE CYP11A1 GENE AND POLYCYSTIC OVARY SYNDROME IN EGYPTIAN FEMALE19285937510.21608/rjab.2016.59375ENWael S.Abdel-MageedMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat city, Sadat City, Minufiya, EgyptEmad DabousMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat city, Sadat City, Minufiya, EgyptAdel GerguisMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat city, Sadat City, Minufiya, EgyptJournal Article20191115Several studies have reported the association of the SNP rs4077582 in the CYP11 gene with hyperandrogenism, which is one of the clinical manifestations of polycystic ovary syndrome (PCOS). These studies suggest that SNP rs4077582 may be involved in the etiopathogenisis of PCOS. To investigate whether the CYP11 gene SNP rs4077582 polymorphism is associated with the susceptibility to PCOS, we designed a case-controlled association study including 104 individuals. A case-controlled association study including 106 individuals (53 PCOS patients and 53 controls) was performed to assess the association of SNP rs4077582 with PCOS. Genotyping of SNP rs4077582 was conducted by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method that was performed on genomic DNA isolated from blood leucocytes. Results were analyzed in respect to clinical test results. The genotypic distributions of rs4077582 (CC, CT, TT) in the CYP11 gene in women with PCOS (CC, 29 (55.8%); CT, 20 (33.5%); and TT, 3 (5.8%). respectively) were significantly different from that in controls (CC, 44 (84.6%); CT, 6 (11.6%); and TT, 2 (3.8%) respectively) (P = 0.001). The allele frequencies in the PCOs women were: C, 78 (75%); and T, 26 (25%). This also was significantly different from the distribution in non-PCOs women: C, 94 (90.4%); and T, 10 (9.6%) (Table 2). In addition, rs4077582 C > T showed a association with PCOs by logistic regression analysis controlling for confounding factors. Our data suggest that SNP rs4077582 in the CYP11 gene is associated with susceptibility to PCOS.https://rjab.journals.ekb.eg/article_59375_3121f70eed483aa6639cc1f7a6b5bb79.pdfUniversity of Sadat City, Genetic Engineering & Biotechnology Research Institute (GEBRI)Research Journal of Applied Biotechnology2356-94332Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology20161101USE OF COPEPTIN IN DIFFERENTIAL DIAGNOSIS OF (ACS) FOR MALES VS FEMALES IN EMERGENCY DEPARTMEN29365942610.21608/rjab.2016.59426ENHossam A.ShahinNational Heart Institute, Imbaba, Giza, Egypt.Sabah F.El AbdMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat city, Sadat City, Egypt.Emad F.Abdel MaksoudLaboratory department, National Heart Institute, Imbaba, Giza, Egypt.Wael S.Abdel MageedMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat city, Sadat City, Egypt.Journal Article20191115ABSTRACT<br />Difference between men and women in the treatment and outcome of acute coronary syndromes has been demonstrated with increased deaths rates in women. Treatment of ACS must be revised and related to the sex and race of the patient. The aim of this study was to introduce new copeptin cutoff levels in ACS patients depending of gender. Also, to compare copeptin to hs-cTnI, CRP levels to predict time delayed between ACS symptoms onset and admission to ED. Sixty two consecutive patients with ACS presenting to the emergency department of the National Heart Institute, Giza (Egypt) between March and June 2014, were recruited for the study. The present study involved thirty patients with unstable angina (19 male & 11 females), thirty two MI patients (18 males & 14 females) and twenty healthy subjects (10 males &10 females) on admission and after 6 hours. Results revealed significant difference between cardiac levels on admission with higher values in men on admission only for CPP, disappeared after six hours. Only copeptin levels were diagnosing on admission (higher with clear cut off levels either for men or women) compared to that of six hours (decreased and get non-significant differences). Early measurement of the combination of troponin I and copeptin may revolutionize the diagnostic accuracy and therapeutic decision-making in patients with symptoms suggestive of ACS, differentiate between UA or AMI patients on admission. Female patients require reevaluation criteria for treatment and outcome.https://rjab.journals.ekb.eg/article_59426_c024e9b91f7e6534b2ba56c7466273c3.pdfUniversity of Sadat City, Genetic Engineering & Biotechnology Research Institute (GEBRI)Research Journal of Applied Biotechnology2356-94332Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology20161101MOLECULAR EVALUATION OF -75G/A AND +83C/T APOLIPOPROTEIN A-1 GENE POLYMORPHISMS IN NON ALCOHOLIC FATTY LIVER DISEASE37455952710.21608/rjab.2016.59527ENMohamed Y.NasrMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, EgyptRisk El-Baz2Department of Biochemistry, Faculty of Medicine, Mansoura University, Mansoura, Egypt.Hesham S.Abd-ElsabourLaboratory Technology, MUST, Egypt.Amal A.Abd-ElazizMolecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt.Journal Article20191116Apolipoprotein A-1 (APOA1) is the major protein component of High Density Lipoprotein in plasma, promotes cholesterol efflux from tissues to the liver for excretion, it is a cofactor for Lecithin Cholesterolacyl Transferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Therefore, the main objective of the present study was carried out to investigate the association of APOA1 gene polymorphisms among patients with Non Alcoholic Fatty Liver Disease (NAFLD) by using PCR-RFLP analysis. Samples were collected from 100 cases affected with NAFLD as well as 100 healthy samples as a negative control. APOA1 +83C/T gene polymorphism was showed that CC genotype was significantly higher frequency (p=0.0001) in healthy control than cases (70% vs. 10%), CT genotype was significantly higher frequency (p=0.001) in cases than healthy control (65% vs. 25%) and TT genotype was significantly higher frequency (p=0.001) in cases than healthy control (25% vs. 5%). Regarding all types of APOA1 -75G/A gene polymorphism, they were showed that all cases were non-significantly (p>0.05) when compared to healthy control. So, NAFLD among Egyptian was strongly associated with mutations related to APOA1 gene polymorphism especially APOA1 +83C/T gene polymorphism.https://rjab.journals.ekb.eg/article_59527_3751c3e7cac06b5fc5df5e72be8e1a3d.pdfUniversity of Sadat City, Genetic Engineering & Biotechnology Research Institute (GEBRI)Research Journal of Applied Biotechnology2356-94332Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology20161101THE CURATIVE ROLE OF CURCUMIN AND FLAXSEED EXTRACTS IN MICE INOCULATED WITH EAC: BIOCHEMICAL AND HISTOLOGICAL STUDY46595954810.21608/rjab.2016.59548ENOsama M.BadrDepartment of Animal Biotechnology, Genetic Engineering & Biotechnology Institute, University of Sadat City, Sadat City, Minufiya Province, Egypt.Eman M.ShirefaDepartment of Animal Biotechnology, Genetic Engineering & Biotechnology Institute, University of Sadat City, Sadat City, Minufiya Province, Egypt.Omaima A.KhamisDepartment of Animal Biotechnology, Genetic Engineering & Biotechnology Institute, University of Sadat City, Sadat City, Minufiya Province, Egypt.Journal Article20191116This investigation discussed the role of curcumin and flaxseed in defeating induced solid tumours with the subcutaneous inoculation of mice with Ehrlich Ascites Carcinoma (EAC) in a definite volume (3X106 cells). Once the tumours were formed, the mice were divided into four groups (control, tumour untreated, curcumin and flaxseed) groups. Each group contains six mice. By the end of experimentation (28 days) after tumour formation, mice were dissected and histological examination for liver and kidney were performed in all groups. Also, the levels of liver functions (ALT & AST), kidney functions (Urea & Creatinine), oxidant and antioxidant enzymes (MDA, SOD and CAT) were measured in all tested groups. This study could state that Curcumin and flaxseed modulated the histological structures of both liver and kidney close to normal patterns. Also, treatments could modified the elevated values of (ALT, AST, Urea, Creatinine and MDA) and decreased levels of (SOD and CAT) towards the normal values. Treatments with Curcumin and flaxseed also reduced the tumour volumes significantly.https://rjab.journals.ekb.eg/article_59548_6384f891808a456fd7736667080de8de.pdfUniversity of Sadat City, Genetic Engineering & Biotechnology Research Institute (GEBRI)Research Journal of Applied Biotechnology2356-94332Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology20161101REGULATION OF CELLULAR IMMUNE RESPONSE BY NATURALLY OCCURRING PRODUCTS ON HEPATOCELLULAR CARCINOMA60675963310.21608/rjab.2016.59633ENMohammed BanawyDepartment of Molecular Biology ,Genetic Engineering and Biotechnology Research Institute , University of Sadat City, EgyptShaden M.HanafyDepartment of Molecular Biology ,Genetic Engineering and Biotechnology Research Institute , University of Sadat City, EgyptHany KhalilDepartment of Molecular Biology ,Genetic Engineering and Biotechnology Research Institute , University of Sadat City, EgyptJournal Article20191117Hepatocellular carcinoma (HCC) is the most important case of liver cancer disease particularly in Egypt. On the other hand, autophagy plays critical role in cellular immune response in different disease including cancer. Notably autophagosome formation required different proteins interaction for initiation, elongation and maturation steps known as Atg proteins . Additionally, tumor suppressor gene including P53 gene play regulatory role in cells proliferation and cell division, down regulation of P53 gene leads to improvement of carcinogenic cells and enhancement of cell proliferation. Here we aim to investigate the possible connection between P53 gene expression and autophagic machinery on HCC upon treatment with some naturally occurring products using HepG2 cell lines. Our findings indicate that, treatment with Guava leafs and Guava flowers extraction increase the expression profile of P53 gene and reduce autophagosome formation indicated by LC3 gene expression. Conversely, the treatment of guava leafs and guava flower extraction on normal cell (normal melanocyte) stimulate autophagic machinery with neutral influence on P53 expression without any detectable cytotoxic effect. Collectively, the natural product from guava leafs and flowers regulate cell proliferation via inhibition of autophagosome formation associated with activation of p53 signaling pathway in HCC. These findings strongly indicate that the indicated nature products can be attractive candidates in therapeutically strategy for liver cancer development.https://rjab.journals.ekb.eg/article_59633_4782b7a7edd56513fb8ce7dd9a2190d4.pdfUniversity of Sadat City, Genetic Engineering & Biotechnology Research Institute (GEBRI)Research Journal of Applied Biotechnology2356-94332Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology20161101THE EFFECT OF PIOGLITAZONE ON GENOMIC INSTABILITY IN INDUCED DIABETIC RATS68805963610.21608/rjab.2016.59636ENKARAM A.AMEINThe Department of Genetics, Faculty of Agriculture, Assiut University, EgyptMOUSTAFA M.HAMDYThe Department of Pharmacology, Faculty of Medicine, Assiut University, EgyptRANIA A.ABD EL-EMAMThe Department of Pharmacology, Faculty of Medicine, Assiut University, EgyptFIKRY H.OSMANThe Department of Pharmacology, Faculty of Medicine, Assiut University, EgyptJournal Article20191117Thiazolidinediones (TZDs) include a group of antihyperglycemic agents that enhance insulin sensitivity and reduce insulin resistance at sites of insulin action specifically skeletal muscles, liver and adipose tissue. In September 2010, the FDA has launched a safety review of pioglitazone citing preliminary epidemiologic evidence suggesting that pioglitazone may be linked to a higher risk for bladder cancer. Bladder tumors were seen in male rats receiving a clinical dose of pioglitazone. Also, in 2 clinical studies, patients receiving pioglitazone experienced a higher rate of bladder cancer than patients taking other agents (Lewis et al., 2008). In this study, the genotoxic and cytotoxic potential of pioglitazone (20, 40 and 80mg/kg, orally daily for 4 weeks) was evaluated against the nicotinamide (230mg/kg) and streptozotocin (65mg/kg) induced somatic and germinal cells defect using a battery of in vivo cytogenetic assays such as the micronucleus, chromosome aberration, mitotic index and sperm abnormality test in male Wistar rats. The obtained results demonstrated that pioglitazone significantly reduced the diabetes-induced genetic damage in both somatic and germinal cells and improved the cell proliferation changes in somatic cells which was attributed to its antioxidant properties. A significant elevation, however, in polyploidy was noticed in the diabetic rats treated with 80mg/kg of pioglitazone. As numerical chromosomal aberrations have also been associated with tumorigenesis, this observation suggests a possible genotoxic effect of pioglitazone when used for a long period in treating diabetes.<br /><br />https://rjab.journals.ekb.eg/article_59636_ace5d1d0a3cac38212a15465cde253d3.pdfUniversity of Sadat City, Genetic Engineering & Biotechnology Research Institute (GEBRI)Research Journal of Applied Biotechnology2356-94332Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology20161101RNA INTERFERNCE (RNAI) FOR SOME GENES FROM BABESIA BOVIS81925963810.21608/rjab.2016.59638ENMahmoud AbouLailaDepartment of Parasitology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Minoufiya, EgyptNaoaki YokoyamaNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, JapanIkuo IgarashiNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido 080-8555, JapanJournal Article20191117Babesia parasites are responsible for enormous economic losses to the livestock industry worldwide. There is no vaccine available to protect from the infection. RNA interference (RNAi) has emerged as a simple and incisive technique to study gene functions in a variety of organisms. In this study, we evaluated the effects of RNAi by double-stranded RNA (dsRNA) of the DNA gyrase subunits A and B, the DNA-directed RNA polymerase beta subunit (rpo B), and thiostrepton interaction site (ribosomal L11 protein) genes on the in vitro growth of Babesia bovis. The inhibitory effects produced by dsRNAs of DNA gyrase subunit A, DNA gyrase subunit B, thiostrepton interaction site (L11) and the DNA-directed RNA polymerase beta subunit (rpo B1and B2) were compared with the effect of drugs target these genes. RNAi directed towards these genes resulted in inhibition of in vitro growth of the parasite. The RT-PCR showed that parasites treated with dsRNAs of DNA gyrase subunit A, DNA gyrase subunit B, ribosomal L11, and the DNA-directed RNA polymerase beta subunit (rpo B1 and B2) have blocked expression of corresponding endogenous mRNAs of DNA gyrase subunit A, DNA gyrase B, ribosomal L11, and rpo B genes. These results demonstrate that the RNAi technique disrupted gene function in B. bovis and the mechanism of action required further studies to be elucidated.https://rjab.journals.ekb.eg/article_59638_838765f75181f9f41326cfff21316441.pdfUniversity of Sadat City, Genetic Engineering & Biotechnology Research Institute (GEBRI)Research Journal of Applied Biotechnology2356-94332Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology20161101BIOSCREENING OF THE METHANOLIC CRUDE EXTRACT OF ULVA LACTUCA AND ITS WATER FRACTION931005963910.21608/rjab.2016.59639ENAhmed M.El-AnsaryMolecular Biology Department, Genetic engineering and biotechnology research institute (GEBRI), University of Sadat City, Sadat city, EgyptDoaa A.GhareebBiochemistry Department, Faculty of Science, Alexandria University, Alexandria, EgyptShaden M.HanafyMolecular Biology Department, Genetic engineering and biotechnology research institute (GEBRI), University of Sadat City, Sadat city, EgyptJournal Article20191117medicine for various purposes. Recently, aquatic habitats have increasing been shown to provide a rich source of natural bioactive compounds. Green algae Ulva lactuca is a widespread macro algae occurring at all levels of the intertidal zone, in calm and protected harbours as deep as 10 meters and in northern climates. Many of its nutrients include iron, protein (15%), iodine, vitamins (A, B1, and C) and trace elements. The present study investigated the bioactivity of Ulva lactuca methanolic crude extract and its water fraction. Also we identified on their different bioactive constituents using high performance liquid chromatography technique. Our results showed that methanolic crude extract of Ulva lactuca and its water fraction had phenolic and flavonoid active constituents acting in synergism to increase their bioactivity such as antioxidant, antiinflamatory and antihemolytic activities. Through 15 days, there was no mortality detected among mice injected with Ulva water fraction different doses. Therefore, we could not calculate the LD50 and accounted it as safe agent. Finally we concluded that Ulva water fraction safe to be used in the treatment of oxidative stress and inflammation related disorders.https://rjab.journals.ekb.eg/article_59639_67ce6891efcd7e9df06f678d98667e3b.pdfUniversity of Sadat City, Genetic Engineering & Biotechnology Research Institute (GEBRI)Research Journal of Applied Biotechnology2356-94332Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology20161101THE ROLE OF BCL-2 ONCOPROTEIN IN HEPATITIS C-RELATED HEPATOCELLULAR CARCINOMA1011105964110.21608/rjab.2016.59641ENGehan, M.IbrahimMolecular biology Dept., Genetic engineering and biotechnology research institute
University of Sadat City, Egypt.Shaden MuawiaMolecular biology Dept., Genetic engineering and biotechnology research institute
University of Sadat City, Egypt.Mohamed El-ShahatMolecular biology Dept., Genetic engineering and biotechnology research institute
University of Sadat City, Egypt.Tarek KamelMolecular biology Dept., Genetic engineering and biotechnology research institute
University of Sadat City, Egypt.Journal Article20191117Viral hepatitis has emerged as a major public health problem worldwide. There is increasing evidence that, several viral oncogenes such as core protein C virus (HCV) is playing a direct role in neoplastic transformation. The tumor development requires both increased proliferation and inhibition of apoptosis. There is increasing experimental evidence to suggest that bcl-2 plays a critical role in blocking apoptosis in cancer cells. In the present study we tried to explore the role of bcl-2 in development of viral hepatitis into hepatocellular carcinoma (HCC). Out of 105 participants; 50 patients were HCV positive infected patients, 30 HCC patients either infected with HCV (HCC/HCV) or not (HCC) and 25 healthy volunteers were also included. We determined the serum level of bcl-2, AFP and HCV-RNA viral load in patients and compared them with the other clinicopathological parameters. Increased serum bcl-2 level was showed in HCC/HCV infected patients more than HCV and controls (P=0.001) and more than HCC patients (P=0.018). A significant positive correlation between AFP and total load of viremia was elucidated (r=0.345, P=0.014) in the HCV infected patients who didn′t develop HCC. By analyzing the diagnostic performance of the studied parameters among the different groups. Bcl-2 was found to be a significant prognostic marker for the progression of HCC, as it represented two or more diagnostic values (Sensitivity 80 % , 70% and specificity 60%, 70% respectively).and (OR=6.0, 95% CI=1.74-20.59& OR=5.4 & 95% CI=1.75-16.88).These results make bcl-2 a candidate marker for HCC progression following HCV infection in Egyptian patients.<br /><br /><br />https://rjab.journals.ekb.eg/article_59641_fd65b82baacb8b4c3f7107ad49b76979.pdfUniversity of Sadat City, Genetic Engineering & Biotechnology Research Institute (GEBRI)Research Journal of Applied Biotechnology2356-94332Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology20161101DIAGNOSTIC VALUE OF FIBRONECTIN AND MIR-122 TARGETS AN ANTI-APOPTOTIC GENE IN EGYPTIAN PATIENTS WITH HEPATOCELLULAR CARCINOMA AND CHRONIC HEPATITIS1111275964210.21608/rjab.2016.59642ENShaden H.MuawiaDepartment of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, Sadat City University, EgyptHala H.El-SaidDepartment of Clinical Biochemistry, National Liver Institute, Menofiya University, EgyptAshraf Y.ElfertDepartment of Clinical Biochemistry, National Liver Institute, Menofiya University, EgyptTarek M.KamelDepartment of Molecular Biology, Genetic Engineering and Biotechnology Research Institute, Sadat City University, EgyptJournal Article20191117Circulating miR-122 is commonly deregulated in liver fibrosis and hepatocellular carcinoma. Fibronection (FN) has been related to liver fibrosis in chronic liver disease. This study examined whether circulating levels of miR-122 regulates tumorigenesis in hepatocellular carcinoma by targeting Bcl-w. Also in this study we tried to examine whether circulating level of miR-122 and FN can be used as noninvasive markers for liver injury in Egyptian patients with chronic hepatitis C virus infection, in addition tried to explore the potential usefulness of serum miR-122 and FN as a diagnostic markers of HCV-related hepatocellular carcinoma (HCC). Out of 84 participants; 20 patients were HCV positive infected patients, 44 HCC patients infected with HCV, and 20 healthy volunteers were also included. We determined the expression of levels of miR-122 and Bcl-w in serum using (qRT-PCR). In addition, the level of plasma FN was measured quantitatively by (ELISA). Mean miR-122 expression levels were up-regulated in both patient groups compared to control group. Conversely, mean Bcl-w expression levels were down-regulated in both patients groups compared to control group. In addition, mean levels of plasma FN were significantly higher in Patient groups as compared to control group (p=<0.001). Furthermore, expression levels of miR-122 and FN were positively correlated with ALT, AST, ALP and fibrosis stage, and negatively correlated with prothrombin concentration and Albumin in both patient groups. By analyzing the diagnostic performance of the studied parameters among patient groups; the combined use of FN and miR-122 achieved (sensitivity 84 % and specificity 70%) for the differentiation of HCC from HCV patients and (sensitivity 81 % and specificity 77%) for the differentiation of patients with early fibrosis from those with significant fibrosis. Conclusion: This study shed light on the functions of miR-122 which may act as an endogenous apoptosis regulator and thus negatively regulates tumorigenesis. Also this study concluded that miR-122 and FN can be used as novel biomarkers for liver injury and may be used to discriminate patients with HCC from HCV.<br /><br />https://rjab.journals.ekb.eg/article_59642_b401f978af559c2b24d21ccdd8a47b81.pdfUniversity of Sadat City, Genetic Engineering & Biotechnology Research Institute (GEBRI)Research Journal of Applied Biotechnology2356-94332Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology20161101BIOLOGICAL ACTIVITY OF MINIMAL DOSE OF AEROSOLIZED INTERFERON-α IN MALE ALBINO RATS1281335964310.21608/rjab.2016.59643ENKhalil A.El-HalfawyMolecular Biology Department, Genetic Engineering and Biotechnology Institute, University of Sadat City, EgyptShaden M.HanafyMolecular Biology Department, Genetic Engineering and Biotechnology Institute, University of Sadat City, EgyptBahgat A.EL-FikyAnimal Biotechnology Department, Genetic Engineering and Biotechnology Institute, University of Sadat City, Egypt.Hassan M.HassanMolecular Biology Department, Genetic Engineering and Biotechnology Institute, University of Sadat City, EgyptJournal Article20191117buffy coat leukocytes Interferon-α (IFN-α) is a leucocyte-derived cytokine with pleiotropic effects on the cells of the immune system, including the ability to promote viral and microbial killing.Five batches of buffy coat leukocytes IFN-α were prepared and purifiied from five male buffalos under the same conditions of collections ; before,during and after slaughter . Three IFN-α doses; 200.500 and 1000 IU were prepared. The present study was designed to evaluate the biologically active dosage of aerosolized buffy coat leukocytes IFN-α, in male albino rats. Four healthy groups were included: Group 1 (control, n=10) received 0 IFN- α ,Group 2 (n=10) received 200 IU IFN-α , Group 3 received 500 IU and Group(4) received 1000 IU / day in order to determine the minimal IFN-α dose able to induce biological effects without side-effects. Result data showed that 500 IFN- α IU/ day for five days induced a significant biological activity, increasing serum levels of biochemical , hematological and IFN-γ levels. It is concluded that aerosolized IFN-α administration to the lung is well-tolerated at biologically active doses. https://rjab.journals.ekb.eg/article_59643_897f99116a5ba3b9aaae8fbf1105f7f7.pdfUniversity of Sadat City, Genetic Engineering & Biotechnology Research Institute (GEBRI)Research Journal of Applied Biotechnology2356-94332Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology20161101BIOSYNTHETICAL CAPACITY OF KAEMPFEROL FROM IN VITRO PRODUCED ARGEL (SOLENOSTEMMA ARGEL (DEL.) HAYNE) CALLUS1341435964410.21608/rjab.2016.59644ENMohamed R.Abd AlhadyTissue Culture Unit, Genetic Resources Department, Ecology and Dry Land Agriculture Division, Desert Research Center, 11753 El-Matariya, 1 Mathaf El-Matariya St., Cairo, EgyptGhada A.HegaziTissue Culture Unit, Genetic Resources Department, Ecology and Dry Land Agriculture Division, Desert Research Center, 11753 El-Matariya, 1 Mathaf El-Matariya St., Cairo, EgyptReda E.Abo El-FadlTissue Culture Unit, Genetic Resources Department, Ecology and Dry Land Agriculture Division, Desert Research Center, 11753 El-Matariya, 1 Mathaf El-Matariya St., Cairo, EgyptSamar Y.DesoukeyPharmacognosy Department, Faculty of Pharmacy, Minia University, 61519 Minia, EgyptJournal Article20191117Solenostemma argel (Del.) Hayne (family Asclepiadaceae) is an Egyptian natural perennial shrub. The study attempted to establish callus from S. argel and to investigate its biosynthetic potentiality to produce the flavonoid kaempferol, which has a wide range of pharmacological activities. Seeds and aerial parts were collected from naturally grown plants at Saint Catherine, Sinai. Callus from different explants of in vitro germinated seedlings (stem, leaf and root) was successfully initiated on Murashige and Skoog (MS) medium supplemented with each of 2,4-dichlorophenoxyacetic acid (2,4-D) and β-naphthalene acetic acid (NAA), separately, at concentrations of 1.0, 2.0 and 3.0 mg/L, in addition to 0.5 mg/L kinetin (KIN). However, the fresh weights of leaf-derived callus were the highest. Casein hydrolysate (CH) and yeast extract (YE), as elicitors, at concentrations of 0.5, 1.0 and 2.0 g/L, were examined for elevating the in vitro production of kaempferol in callus cultures. The total methanol extracts of the aerial parts of wild plants, four-week-old in vitro germinated seedlings’ explants and their derived callus were analyzed qualitatively and quantitatively for kaempferol, using HPLC. A significant difference in the content of kaempferol was observed. Callus produced from tested explants showed a capacity for production and accumulation of kaempferol. The highest concentration of kaempferol was in callus obtained from seedlings’ stem explants on MS medium containing 1.0 mg/L 2,4-D and 0.5 mg/L KIN, in addition to 2.0 mg/L YE. In conclusion, S. argel callus cultures showed a capacity for synthesizing kaempferol and could be an alternative renewable natural source for its large-scale production. https://rjab.journals.ekb.eg/article_59644_d50eaa76a435f6bbc5492f83803e32ca.pdf