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Soliman, M., Elhalafawi, K., Ibrahim, I., Mahrous, E., Bekhit, M. (2018). Utilizing RAPD Analysis as Molecular Markers for comparison between Various Callus and Mother Plant of Jatropha curcas L.. Research Journal of Applied Biotechnology, 4(2), 75-85. doi: 10.21608/rjab.2018.106847
Manal S. Soliman; Khallil Elhalafawi; Ibrahim A. Ibrahim; Emad Mahrous; Metwally Bekhit. "Utilizing RAPD Analysis as Molecular Markers for comparison between Various Callus and Mother Plant of Jatropha curcas L.". Research Journal of Applied Biotechnology, 4, 2, 2018, 75-85. doi: 10.21608/rjab.2018.106847
Soliman, M., Elhalafawi, K., Ibrahim, I., Mahrous, E., Bekhit, M. (2018). 'Utilizing RAPD Analysis as Molecular Markers for comparison between Various Callus and Mother Plant of Jatropha curcas L.', Research Journal of Applied Biotechnology, 4(2), pp. 75-85. doi: 10.21608/rjab.2018.106847
Soliman, M., Elhalafawi, K., Ibrahim, I., Mahrous, E., Bekhit, M. Utilizing RAPD Analysis as Molecular Markers for comparison between Various Callus and Mother Plant of Jatropha curcas L.. Research Journal of Applied Biotechnology, 2018; 4(2): 75-85. doi: 10.21608/rjab.2018.106847

Utilizing RAPD Analysis as Molecular Markers for comparison between Various Callus and Mother Plant of Jatropha curcas L.

Article 9, Volume 4, Issue 2, November 2018, Page 75-85  XML PDF (538.69 K)
Document Type: Original Article
DOI: 10.21608/rjab.2018.106847
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Authors
Manal S. Soliman1; Khallil Elhalafawi2; Ibrahim A. Ibrahim1; Emad Mahrous1; Metwally Bekhit email 3
1Plant Biotechnology Dept. Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt.
2Molecular Biology Dept. Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt.
3Plant Biotechnology Dept. Genetic Engineering and Biotechnology Research Institute (GEBRI),University of Sadat City, Egypt.
Abstract
Calli were induced from different explants of Jatropha curcas L. seedling on MS basal agar medium in presence of different plant growth regulators. at different levels for 4 weeks Random Amplified Polymorphic DNA (RAPD) markers were used for the assessment of genetic variation of callus grown in different hormonal combinations in comparison with the mother plant. A total of 8 arbitrary sequence primers were evaluated. Of 8 primers used for the RAPD analysis, primers showed consistent band patterns. In total, scorable bands were observed with the primers. The total number of amplicons produced per primer varied from 5 for OPA-07 and OPB-12 to as many as 8 bands for OPB-10, OPC- 09 and OPR-17. The average number of bands per primer was 6.625. Out of 53 bands, 36 were polymorphic (67.92%). The average number of polymorphic RAPD bands was 4.5 per primer. the highest similarity (0.71) with mother plant was recorded in leaf-derived callus (S4) grown on MS solid medium with 2.5 μM NAA plus 2.5 μM IBA, while endosperm callus maintained on MS with hormonal combination 12.5 μM IBA + BAP- 25.0 μM was found to show least similarity (0.54) with mother plant.
In this concern, results have been suggested to be useful fast methods for comparing of genetic
changes and variation in plants.
Keywords
Jatropha curcas L., Callus induction, Growth regulators, RAPD analysis, Abbreviations: BAP, 6 Benzylaminopurine; IBA, indole-3-butyric acid; 2,4-D, 2,4-dichlorophenoxyacetic acid; MS, Murashige and Skoog; RAPD, Random Amplified Polymorphic DNA; TBE, Tris/Borate/EDTA buffer; WPM, Woody Plant Medium
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