Molecular evaluation of Hepatocyte Growth Factor (HGF) as a potential Biomarker to detect Hepatocellular Carcinoma

Document Type : Original Article

Authors

1 Molecular diagnostics and therapeutics department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat city, Egypt

2 Clinical Pathology Department, National Liver Institute, Menofia University, Menofia, Egypt

Abstract

This study was carried out to investigate the noninvasive expression level of hepatocyte growth factor (HGF) in hepatocellular carcinoma patients and its relationship with diagnostic parameters.To active this goal we used thirty six patient divide to fourteen with HCC, twelve patients with HCV and ten healthy control patient matched in age and sex were investigated. HGF level wasdetected by quantitative polymerase chain reaction (qRT-PCR). The median age of the patients was 51 years (rang 44-58 years); where males constituted the majority of the group (69.4%). The entire patient had cirrhotic history. None of the patients were pretreated with local therapies. The baseline noninvasive HGF level wassignificantly higher in patient HCC than in the HCV and control groups (p=0.028), whereas HGF level was alsohigher in HCV patient than healthy patient. Further the correlation between the level of HGF and all laboratory parameters was tested. We found that there is a good positive relationship between HGF levels and SGPT (ALT), SOGT (AST), Platelets count.Finally we compared the predicative accuracy of HGF and a conventional tumor marker AFP by ROC curve; we found that the sensitivity of HGF (85.7%) to predict the HCC is higher than AFP (73%), and also the specificity.Overall, these results suggest that HGF could serve as a useful tumor biomarker for prediction of HCC in comparing with, HCV, and healthy control results.

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