Agricultural wastes are very rich in nutrients that can be used as complete balanced microbiological media for growing microbes in order to produce valuable commercial products. In this study, a biodegradation process of wheat bran and rice bran wastes was carried out to produce metalloprotease enzyme from B. thuringiensis 1257strain which is considered to be a hyper producer for that enzyme. Metalloprotease was partially purified by ammonium sulfate followed by application on Sephadex G-75 column. Gel filtration step resulted in more than 40 times fold purification of the purified enzyme. The enzyme activity was inhibited by EDTA (almost 80%) at 15 mM concentration. These agro-industrial wastes were used as substrates for economic production of the enzyme from B. thuringiensis compared with some bacterial isolates collected from different sites in Kafr El-Sheikh and Menofiya governorate, Egypt. Optimum temperature for enzyme activity was 40 °C. It also exhibited a broad pH activity range (6-9) with an optimum pH of 8.
Rizk, N., Eldourghamy, A., & Elgamal, E. (2017). BIOREMEDIATION OF SOME AGRICULTURAL WASTES BY BACILLUS THURINGIENSIS FOR METALLOPROTEASE PRODUCTION. Research Journal of Applied Biotechnology, 3(1), 11-22. doi: 10.21608/rjab.2017.57586
MLA
Nashwa M. H. Rizk; Ayman S. Eldourghamy; Eman G. Elgamal. "BIOREMEDIATION OF SOME AGRICULTURAL WASTES BY BACILLUS THURINGIENSIS FOR METALLOPROTEASE PRODUCTION", Research Journal of Applied Biotechnology, 3, 1, 2017, 11-22. doi: 10.21608/rjab.2017.57586
HARVARD
Rizk, N., Eldourghamy, A., Elgamal, E. (2017). 'BIOREMEDIATION OF SOME AGRICULTURAL WASTES BY BACILLUS THURINGIENSIS FOR METALLOPROTEASE PRODUCTION', Research Journal of Applied Biotechnology, 3(1), pp. 11-22. doi: 10.21608/rjab.2017.57586
VANCOUVER
Rizk, N., Eldourghamy, A., Elgamal, E. BIOREMEDIATION OF SOME AGRICULTURAL WASTES BY BACILLUS THURINGIENSIS FOR METALLOPROTEASE PRODUCTION. Research Journal of Applied Biotechnology, 2017; 3(1): 11-22. doi: 10.21608/rjab.2017.57586
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