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Rizk, N., Eldourghamy, A., Elgamal, E. (2017). BIOREMEDIATION OF SOME AGRICULTURAL WASTES BY BACILLUS THURINGIENSIS FOR METALLOPROTEASE PRODUCTION. Research Journal of Applied Biotechnology, 3(1), 11-22. doi: 10.21608/rjab.2017.57586
Nashwa M. H. Rizk; Ayman S. Eldourghamy; Eman G. Elgamal. "BIOREMEDIATION OF SOME AGRICULTURAL WASTES BY BACILLUS THURINGIENSIS FOR METALLOPROTEASE PRODUCTION". Research Journal of Applied Biotechnology, 3, 1, 2017, 11-22. doi: 10.21608/rjab.2017.57586
Rizk, N., Eldourghamy, A., Elgamal, E. (2017). 'BIOREMEDIATION OF SOME AGRICULTURAL WASTES BY BACILLUS THURINGIENSIS FOR METALLOPROTEASE PRODUCTION', Research Journal of Applied Biotechnology, 3(1), pp. 11-22. doi: 10.21608/rjab.2017.57586
Rizk, N., Eldourghamy, A., Elgamal, E. BIOREMEDIATION OF SOME AGRICULTURAL WASTES BY BACILLUS THURINGIENSIS FOR METALLOPROTEASE PRODUCTION. Research Journal of Applied Biotechnology, 2017; 3(1): 11-22. doi: 10.21608/rjab.2017.57586

BIOREMEDIATION OF SOME AGRICULTURAL WASTES BY BACILLUS THURINGIENSIS FOR METALLOPROTEASE PRODUCTION

Article 9, Volume 3, Issue 1, June 2017, Page 11-22  XML PDF (322.35 K)
Document Type: Original Article
DOI: 10.21608/rjab.2017.57586
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Authors
Nashwa M. H. Rizk; Ayman S. Eldourghamy email ; Eman G. Elgamal
Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat city, Egypt
Abstract
Agricultural wastes are very rich in nutrients that can be used as complete balanced microbiological media for growing microbes in order to produce valuable commercial products. In this study, a biodegradation process of wheat bran and rice bran wastes was carried out to produce metalloprotease enzyme from B. thuringiensis 1257strain which is considered to be a hyper producer for that enzyme. Metalloprotease was partially purified by ammonium sulfate followed by application on Sephadex G-75 column. Gel filtration step resulted in more than 40 times fold purification of the purified enzyme. The enzyme activity was inhibited by EDTA (almost 80%) at 15 mM concentration. These agro-industrial wastes were used as substrates for economic production of the enzyme from B. thuringiensis compared with some bacterial isolates collected from different sites in Kafr El-Sheikh and Menofiya governorate, Egypt. Optimum temperature for enzyme activity was 40 °C. It also exhibited a broad pH activity range (6-9) with an optimum pH of 8.
Keywords
metalloprotease; B. thuringiensis1257; EDTA; agro-industrial waste; Sephadex G-75
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