ISOLATION AND IDENTIFICATION OF HALOALKALIPHILIC HALOMONAS SP. HA1 FROM WADI EL NATRUN LAKES, EGYPT

Elsayed, Afaf; Abbas, Nasser H.; Hassan, Hamdy A.; M. A., Sabha

doi:10.21608/rjab.2017.57646

ISOLATION AND IDENTIFICATION OF HALOALKALIPHILIC HALOMONAS SP. HA1 FROM WADI EL NATRUN LAKES, EGYPT

Document Type : Original Article

Authors

¹ Botany Department, Faculty of Science, Menoufia University, Menoufia, Egypt

² Genetic Engineering and Biotechnology Research Institute, Molecular Biology Department, University of Sadat City, Sadat City, Egypt.

10.21608/rjab.2017.57646

Abstract

Haloalkaliphilic bacterial strain was isolated from Hamra lake in Wdi EL-Natrun area. The 16s rRNA sequence of this strain shows a phylogenic relationship with Gammaproteobacteria family Halomondaceae. The strain was registered in gene bank as Halomonas HA1 strain with accession number KT223026. This strain show obligatory NaCl requirements for growth. The optimum NaCl concentration was 8% and the maximum NaCl concentration tolerance was 20%. Halomonas HA1 show extremely alkaliphilic growth pattern with optimum growth at pH 9.0 and the maximum tolerant was at pH 11.

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