El-Halfawy, K., Hanafy, S., EL-Fiky, B., Hassan, H. (2016). BIOLOGICAL ACTIVITY OF MINIMAL DOSE OF AEROSOLIZED INTERFERON-α IN MALE ALBINO RATS. Research Journal of Applied Biotechnology, 2(Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology), 128-133. doi: 10.21608/rjab.2016.59643
Khalil A. El-Halfawy; Shaden M. Hanafy; Bahgat A. EL-Fiky; Hassan M. Hassan. "BIOLOGICAL ACTIVITY OF MINIMAL DOSE OF AEROSOLIZED INTERFERON-α IN MALE ALBINO RATS". Research Journal of Applied Biotechnology, 2, Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology, 2016, 128-133. doi: 10.21608/rjab.2016.59643
El-Halfawy, K., Hanafy, S., EL-Fiky, B., Hassan, H. (2016). 'BIOLOGICAL ACTIVITY OF MINIMAL DOSE OF AEROSOLIZED INTERFERON-α IN MALE ALBINO RATS', Research Journal of Applied Biotechnology, 2(Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology), pp. 128-133. doi: 10.21608/rjab.2016.59643
El-Halfawy, K., Hanafy, S., EL-Fiky, B., Hassan, H. BIOLOGICAL ACTIVITY OF MINIMAL DOSE OF AEROSOLIZED INTERFERON-α IN MALE ALBINO RATS. Research Journal of Applied Biotechnology, 2016; 2(Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology): 128-133. doi: 10.21608/rjab.2016.59643
BIOLOGICAL ACTIVITY OF MINIMAL DOSE OF AEROSOLIZED INTERFERON-α IN MALE ALBINO RATS
1Molecular Biology Department, Genetic Engineering and Biotechnology Institute, University of Sadat City, Egypt
2Animal Biotechnology Department, Genetic Engineering and Biotechnology Institute, University of Sadat City, Egypt.
Abstract
buffy coat leukocytes Interferon-α (IFN-α) is a leucocyte-derived cytokine with pleiotropic effects on the cells of the immune system, including the ability to promote viral and microbial killing.Five batches of buffy coat leukocytes IFN-α were prepared and purifiied from five male buffalos under the same conditions of collections ; before,during and after slaughter . Three IFN-α doses; 200.500 and 1000 IU were prepared. The present study was designed to evaluate the biologically active dosage of aerosolized buffy coat leukocytes IFN-α, in male albino rats. Four healthy groups were included: Group 1 (control, n=10) received 0 IFN- α ,Group 2 (n=10) received 200 IU IFN-α , Group 3 received 500 IU and Group(4) received 1000 IU / day in order to determine the minimal IFN-α dose able to induce biological effects without side-effects. Result data showed that 500 IFN- α IU/ day for five days induced a significant biological activity, increasing serum levels of biochemical , hematological and IFN-γ levels. It is concluded that aerosolized IFN-α administration to the lung is well-tolerated at biologically active doses.
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