Gene polymorphism of Il-18 as a pro-inflammatory mediator and susceptibility of diabetes type 1in Egyptian children.

Document Type : Original Article

Authors

1 Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt.

2 National Institute of Diabetes and Endocrinology (NIDE), Cairo University, Egypt

Abstract

Type 1 diabetes (T1D) is a chronic immune-mediated disease and is the most common autoimmune disorder in childhoodbut the disease may become manifest at any age. It is characterized by selective loss of insulin-producing β-cells in the pancreatic islets in genetically susceptible subjects.Interleukin- 18 (IL-18) is a proinflammatory cytokinethat was originally identified as an interferon-γ inducing factor.It plays a critical role in chronic inflammation, in autoimmune diseases, and in a number of infectious diseases. Functional variants of IL18 gene has been reported as associated with T1D.the purpose of this was to investigate the role of IL-18 gene polymorphism (-607 A/C)in the prediction of T1D in the Egyptian children. One hundred and four patients with T1D and 116 healthy controls, IL- 18 promoter genotyping at -607 (rs1946518) position was analyzed with sequence-specific PCR(PCR/SSP).Therewas no significant association between polymorphisms in SNPs of IL-18 (rs1946518A/C) and T1D risk in Egyptian population,However, the results suggest an association of genotypes with age at onset of T1D.Our preliminary data suggested that polymorphisms in the IL-18 gene may not contribute to T1D susceptibility. To the best of our knowledge, this study is the first one that deals with IL-18 gene polymorphism in T1D patients in Egypt. Although, further studies with large sample size should be conducted to validate these results in Egyptian population.

Keywords

Research Journal of Applied Biotechnology
Volume 4, Issue 2
November 2018
Pages 20-29

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