Eldourghamy, A., Elsabagh, S., Ismail, A. (2015). Biodegradation of diesel oil wastes for production of biosurfactants by two Actinomycetes strains. Research Journal of Applied Biotechnology, 1(1), 64-79. doi: 10.21608/rjab.2015.53532
Ayman Eldourghamy; Sabha Elsabagh; Abdallah Ismail. "Biodegradation of diesel oil wastes for production of biosurfactants by two Actinomycetes strains". Research Journal of Applied Biotechnology, 1, 1, 2015, 64-79. doi: 10.21608/rjab.2015.53532
Eldourghamy, A., Elsabagh, S., Ismail, A. (2015). 'Biodegradation of diesel oil wastes for production of biosurfactants by two Actinomycetes strains', Research Journal of Applied Biotechnology, 1(1), pp. 64-79. doi: 10.21608/rjab.2015.53532
Eldourghamy, A., Elsabagh, S., Ismail, A. Biodegradation of diesel oil wastes for production of biosurfactants by two Actinomycetes strains. Research Journal of Applied Biotechnology, 2015; 1(1): 64-79. doi: 10.21608/rjab.2015.53532
Biodegradation of diesel oil wastes for production of biosurfactants by two Actinomycetes strains
1Environmental Biotechnology Department, GEBRI, University of Sadat City
2Department of Microbiology, Faculty of Science, University of Minufiya
Abstract
Biotechnological studies on production of bio-surfactants by actinomycetes strains that were isolated from Egyptian soil were carried out using some diesel oil waste samples. Two actinomycetes strains were found to give bio-surfactant production up to 40 - % when tested with emulsification test. Effect of carbon and nitrogen sources on bio-surfactant production were tested using urea, ammonium nitrate, ammonium chloride and potassium nitrate. Best results for bio-surfactant production were observed with urea and ammonium nitrate as the main nitrogen source in the biological medium. Best results for bio-surfactant production were observed when using Bushnell Hass medium supplement with % hydrocarbon waste. Ammonium sulfate precipitated samples were further applied on Sephadex LH-20 column to obtain purified products. Results obtained from isolated strains show optimistic results for a successful bioremediation of diesel oil wastes for production of commercial active bio-surfactants that could be used in both industrial and pharmaceutical fields.
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