Effect of glutathione on antimicrobial activity of levofloxacin

AbdEl-Aziz, Amal A.; Zahran, Wafaa A.; Mahrous, Hoda; Khalil, Hany; El-Khayat, Amira H.; El-Nahas, Saadiya A.

doi:10.21608/rjab.2018.57524

Effect of glutathione on antimicrobial activity of levofloxacin

Document Type : Original Article

Authors

¹ Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat city, Egypt

² Microbiology and Immunology Department, faculty of Medicine, Menofiya University, Menofiya, Egypt

³ Industerial Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat city, Egypt

10.21608/rjab.2018.57524

Abstract

Overuse of antibiotics has become the major factor for the emergence anddissemination of multi-drug resistant strains of several groups of microorganisms andthis lead to search for agents that may be asolution,Fluoroquinolones are a group of antibiotics widelyused because of their broad spectrum activity against both Gram-positive and Gram-negative bacteria.
In this study We report the effect of glutathione on the antibacterial action of levofloxacin on E-coli and S. aureus aslevofloxacin is an important and commonly used members of the fluoroquinolonesantibiotics.Itinhibit DNA topoisomerase II and DNA topoisomerase IV activities, eventually leading tobacterial cell death. In addition, an increase of reactive oxygen species in the bacterial cells in response to levofloxacin has been shown.

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