MOLECULAR EVALUATION OF -75G/A AND +83C/T APOLIPOPROTEIN A-1 GENE POLYMORPHISMS IN NON ALCOHOLIC FATTY LIVER DISEASE

Document Type : Original Article

Authors

1 Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt

2 2Department of Biochemistry, Faculty of Medicine, Mansoura University, Mansoura, Egypt.

3 Laboratory Technology, MUST, Egypt.

4 Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt.

Abstract

Apolipoprotein A-1 (APOA1) is the major protein component of High Density Lipoprotein in plasma, promotes cholesterol efflux from tissues to the liver for excretion, it is a cofactor for Lecithin Cholesterolacyl Transferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Therefore, the main objective of the present study was carried out to investigate the association of APOA1 gene polymorphisms among patients with Non Alcoholic Fatty Liver Disease (NAFLD) by using PCR-RFLP analysis. Samples were collected from 100 cases affected with NAFLD as well as 100 healthy samples as a negative control. APOA1 +83C/T gene polymorphism was showed that CC genotype was significantly higher frequency (p=0.0001) in healthy control than cases (70% vs. 10%), CT genotype was significantly higher frequency (p=0.001) in cases than healthy control (65% vs. 25%) and TT genotype was significantly higher frequency (p=0.001) in cases than healthy control (25% vs. 5%). Regarding all types of APOA1 -75G/A gene polymorphism, they were showed that all cases were non-significantly (p>0.05) when compared to healthy control. So, NAFLD among Egyptian was strongly associated with mutations related to APOA1 gene polymorphism especially APOA1 +83C/T gene polymorphism.

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