Nasr, M., El-Baz, R., Abd-Elsabour, H., Abd-Elaziz, A. (2016). MOLECULAR EVALUATION OF -75G/A AND +83C/T APOLIPOPROTEIN A-1 GENE POLYMORPHISMS IN NON ALCOHOLIC FATTY LIVER DISEASE. Research Journal of Applied Biotechnology, 2(Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology), 37-45. doi: 10.21608/rjab.2016.59527
Mohamed Y. Nasr; Risk El-Baz; Hesham S. Abd-Elsabour; Amal A. Abd-Elaziz. "MOLECULAR EVALUATION OF -75G/A AND +83C/T APOLIPOPROTEIN A-1 GENE POLYMORPHISMS IN NON ALCOHOLIC FATTY LIVER DISEASE". Research Journal of Applied Biotechnology, 2, Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology, 2016, 37-45. doi: 10.21608/rjab.2016.59527
Nasr, M., El-Baz, R., Abd-Elsabour, H., Abd-Elaziz, A. (2016). 'MOLECULAR EVALUATION OF -75G/A AND +83C/T APOLIPOPROTEIN A-1 GENE POLYMORPHISMS IN NON ALCOHOLIC FATTY LIVER DISEASE', Research Journal of Applied Biotechnology, 2(Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology), pp. 37-45. doi: 10.21608/rjab.2016.59527
Nasr, M., El-Baz, R., Abd-Elsabour, H., Abd-Elaziz, A. MOLECULAR EVALUATION OF -75G/A AND +83C/T APOLIPOPROTEIN A-1 GENE POLYMORPHISMS IN NON ALCOHOLIC FATTY LIVER DISEASE. Research Journal of Applied Biotechnology, 2016; 2(Special issue (1) for the first International Conference of Genetic Engineering and Biotechnology): 37-45. doi: 10.21608/rjab.2016.59527
MOLECULAR EVALUATION OF -75G/A AND +83C/T APOLIPOPROTEIN A-1 GENE POLYMORPHISMS IN NON ALCOHOLIC FATTY LIVER DISEASE
1Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt
22Department of Biochemistry, Faculty of Medicine, Mansoura University, Mansoura, Egypt.
3Laboratory Technology, MUST, Egypt.
4Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Egypt.
Abstract
Apolipoprotein A-1 (APOA1) is the major protein component of High Density Lipoprotein in plasma, promotes cholesterol efflux from tissues to the liver for excretion, it is a cofactor for Lecithin Cholesterolacyl Transferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. Therefore, the main objective of the present study was carried out to investigate the association of APOA1 gene polymorphisms among patients with Non Alcoholic Fatty Liver Disease (NAFLD) by using PCR-RFLP analysis. Samples were collected from 100 cases affected with NAFLD as well as 100 healthy samples as a negative control. APOA1 +83C/T gene polymorphism was showed that CC genotype was significantly higher frequency (p=0.0001) in healthy control than cases (70% vs. 10%), CT genotype was significantly higher frequency (p=0.001) in cases than healthy control (65% vs. 25%) and TT genotype was significantly higher frequency (p=0.001) in cases than healthy control (25% vs. 5%). Regarding all types of APOA1 -75G/A gene polymorphism, they were showed that all cases were non-significantly (p>0.05) when compared to healthy control. So, NAFLD among Egyptian was strongly associated with mutations related to APOA1 gene polymorphism especially APOA1 +83C/T gene polymorphism.
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