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Rizk, I., Amaar, M., Ibrahim, I. (2017). Protein Analysis of Marjoram Plants Treated with Biocontrol Agents. Research Journal of Applied Biotechnology, 3(2), 1-6. doi: 10.21608/rjab.2017.104549
Islam M. H. Rizk; Mohamed M. M. Amaar; Ibrahim A. Ibrahim. "Protein Analysis of Marjoram Plants Treated with Biocontrol Agents". Research Journal of Applied Biotechnology, 3, 2, 2017, 1-6. doi: 10.21608/rjab.2017.104549
Rizk, I., Amaar, M., Ibrahim, I. (2017). 'Protein Analysis of Marjoram Plants Treated with Biocontrol Agents', Research Journal of Applied Biotechnology, 3(2), pp. 1-6. doi: 10.21608/rjab.2017.104549
Rizk, I., Amaar, M., Ibrahim, I. Protein Analysis of Marjoram Plants Treated with Biocontrol Agents. Research Journal of Applied Biotechnology, 2017; 3(2): 1-6. doi: 10.21608/rjab.2017.104549

Protein Analysis of Marjoram Plants Treated with Biocontrol Agents

Article 1, Volume 3, Issue 2, November 2017, Page 1-6  XML PDF (488.09 K)
Document Type: Original Article
DOI: 10.21608/rjab.2017.104549
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Authors
Islam M. H. Rizk email 1; Mohamed M. M. Amaar2; Ibrahim A. Ibrahim3
1Department of Environmental Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat city, Sadat city, Egypt
2Department of Agricultural Botany, Faculty of Agriculture, Minufiya University, Egypt
3Department of Plant Biotechnology Genetic Engineering and Biotechnology Research Institute,University of Sadat City, Sadat City
Abstract
Origanum majorana L. commonly known as sweet marjoram has been used for variety of diseases in traditional medicine, including gastrointestinal, ocular, nasopharyngeal, respiratory, cardiac, rheumatologic, and neurological disorders. Protein gel electrophoresis is the commonly used technology to separate proteins according to their physical properties such as electrical charge and molecular weight.In present study, polyacrylamide gel electrophoresis (SDS-PAGE) was used for identification of marjoram plants treated with biocontrol agents collected from Egypt and studying the differentiation among them.SDS-PAGE was examined for plants treated with four biocontrol agents;Bacillus megaterium,Fusarium aquaeductuum,Trichoderma harzinum+Fusarium aquaeductuum and Bacillus megaterium+Trichoderma harzinum +Trichoderma hamatum+Fusarium aquaeductuum.Protein analysis revealed that, application of the biocontrol agents significantly increased protein bands than control or plants cultivated in the infested soil, best results were obtained when B. megaterium was applied.
 
Keywords
Origanum majorana; traditional medicine; Fusarium aquaeductuum; polyacrylamide gel electrophoresis (SDS-PAGE); Majorana hortensis
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